transcriptomics

Transcriptional response of the harmful raphidophyte Heterosigma akashiwo to nitrate and phosphate stress

The marine eukaryotic alga *Heterosigma akashiwo* (Raphidophyceae) is known for forming ichthyotoxic harmful algal blooms (HABs). In the past 50 years, *H. akashiwo* blooms have increased, occurring globally in highly eutrophic coastal and estuarine …

Conserved transcriptional responses to nutrient stress in bloom-forming algae

The concentration and composition of bioavailable nitrogen (N) and phosphorus (P) in the upper ocean shape eukaryotic phytoplankton communities and influence their physiological responses. Phytoplankton are known to exhibit similar physiological …

Phosphorus availability regulates intracellular nucleotides in marine eukaryotic phytoplankton

Marine eukaryotic phytoplankton adapt to low phosphorus (P) in the oceans through a variety of step‐wise mechanisms including lipid substitution and decreased nucleic acid content. Here, we examined the impact of low P concentrations on intracellular …

Probing the evolution, ecology and physiology of marine protists using transcriptomics

Protists, which are single-celled eukaryotes, critically influence the ecology and chemistry of marine ecosystems, but genome-based studies of these organisms have lagged behind those of other microorganisms. However, recent transcriptomic studies of …

Identifying reference genes with stable expression from high throughput sequence data

Genes that are constitutively expressed across multiple environmental stimuli are crucial to quantifying differentially expressed genes, particularly when employing quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) assays. …

The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response

Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on …