de novo assembly

Re-assembly, quality evaluation, and annotation of 678 microbial eukaryotic reference transcriptomes

**Background** De novo transcriptome assemblies are required prior to analyzing RNAseq data from a species without an existing reference genome or transcriptome. Despite the prevalence of transcriptomic studies, the effects of using different …

Shifting metabolic priorities among key protistan taxa within and below the euphotic zone

A metatranscriptome study targeting the protistan community was conducted off the coast of Southern California, at the San Pedro Ocean Time‐series station at the surface, 150 m (oxycline), and 890 m to link putative metabolic patterns to distinct …

Transcriptional response of the harmful raphidophyte Heterosigma akashiwo to nitrate and phosphate stress

The marine eukaryotic alga *Heterosigma akashiwo* (Raphidophyceae) is known for forming ichthyotoxic harmful algal blooms (HABs). In the past 50 years, *H. akashiwo* blooms have increased, occurring globally in highly eutrophic coastal and estuarine …